Facts-enriched characterization of mobile populations. Morphological data from pictures can incorporate to your richness of flow cytometry info. One example is, the determine reveals an or else common apoptosis assay using Annexin V and PI, incorporating cell imaging to characterize cells in each inhabitants to expose morphologically distinctive features. These insights couldn't have already been gained from multiplex staining alone.
As samples are obtained on the Attune CytPix Flow Cytometer, the higher-velocity brightfield camera captures and merchants illustrations or photos of detected situations in a rate as many as 6,000 images for every second, with regards to the flow charge and impression dimension. For higher flexibility, Attune Cytometric Software package allows you to regulate the graphic capture frequency as essential and choose a selected gate for impression seize, if needed.
Merge acoustic concentrating engineering with large-parameter Evaluation for flexible spectral unmixing and standard workflows.
The award-successful Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating by using a higher-pace brightfield digicam for simultaneous superior throughput flow cytometry and higher resolution brightfield imaging.
*Pricing is indicative only and it is subject matter to alter. Make sure you contact your Agilent consultant with any concerns. The data established forth herein is just not a proposal or possibly a determination, the two of which might be topic to credit history approval, terms and conditions, and availability with Agilent. Agilent Fiscal Alternatives is out there in picked nations.
In this article, a skilled user has gated singlets confidently. Right after assessing the handbook singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate consists of over four% aggregates.
Pair your Attune flow cytometer by having an autosampler for increased workflow effectiveness, and include robotic integration for max throughput.
Our scientists will layout panels for you as many times you may need—totally free, no product obtain wanted.
Early indications of this contamination had in the beginning been dismissed as particles. Backgating to find out new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also helps you to use morphological capabilities to find out interesting subpopulations that will not be evident from flow cytometry data by itself.
Most likely most of all, these functions have cells of Plainly various phenotypes which could lead to incorrect conclusions with regards to double constructive functions (particularly in exceptional populations).
Sound waves permit exact cell alignment, leading to Fantastic facts top quality and ~10X quicker sample flow rates.
Application note: Automatic impression analysis lowers user-to-consumer variability in flow cytometry gating approaches
Leverage the power of Highly developed technologies and innovation to speed up your discoveries. Rely on from the Attune Flow Cytometer relatives to deliver the reliability, pace, https://www.metooo.it/u/67d3e4f36d8dfb4e83688058 and precision that established your get the job done apart.
Versatile payment plan from Agilent Economical Answers enable you to obtain the essential instrumentation you'll need for immediate challenge, or to grow your lab.
Gating on these extended parameters helps you to rapidly and properly recognize populations of fascination and ensure gating strategy with little or no handbook overview. The Attune program offers usage of state-of-the-art equipment Understanding Assessment instruments inside of an expertly made user interface, rendering it extremely simple to use.
The Attune Xenith cytometer is a substantial-parameter spectral analyzer, giving both spectral unmixing and standard flow abilities for detailed and versatile mobile Investigation.
The Attune CytPix Flow Cytometer permits users to visualise samples during acquisition, validate the precision of gating procedures, and help benefits with reliable documentation for publication needs.
In this article, we display that by gating strategies using impression-primarily based quantitative parameters can distinguish interacting cells from coincident events to much more precisely assess interacting cells.